The invention relates to bio-
organic fertilizer prepared by using
agrobacterium rhizogenes and a preparation method of the bio-
organic fertilizer. The bio-
organic fertilizer is prepared from main rawmaterials of A4, R1205, R1000, R1601, R1022 and 15834
agrobacterium rhizogenes. Through the
agrobacterium rhizogenes, an effect of promotion of
root growth of plants can be achieved, and an importantinfluence on the promotion of the growth and development of the plants can be generated. Therefore, the bio-organic
fertilizer is prepared from an agrobacterium rhizogenes solution with an OD value of0.4-0.6, an
organic matter composition and
biomass charcoal; after the application of the bio-organic
fertilizer to the plants, nutrients needed for the growth of the plants can be met, the abilitiesof
drought resistance and
disease resistance of the plants can be improved, and the growth of root systems of the plants can be stimulated so as to increase the absorption area of the root systems, so that the rooting ability of the plants is enhanced, the
nutrient efficiency of the plants is improved, and the growth and development of the plants are promoted; the problems of low quality and lowyield in
plant production can be solved, and the improvement of potential
economic benefits of the plants and agricultural production are facilitated.